Biochemistry Seminar: Frank Robb
Wed, Oct 30
12:00 PM — 1:00 PM
Marshak160 Convent Avenue
Frank Robb will give a talk titled "Phylogeny and Mechanism for Stabilizing the Chaperonin, Cpn60."Frank Robb, Professor, Institute of Marine & Environmental Technology, Dept of Microbiology & Immunology, University of Maryland School of Medicine, will give a talk titled, "Phylogeny and Mechanism for Stabilizing the Chaperonin, Cpn60."
Chaperonins are universal proteins that are critical for cell survival, especially in extreme thermophiles. A longstanding convention divides Cpns into two groups that share many conserved sequence motifs but differ in both subunit structure and distribution. We discovered a third, distinct group of Cpn in divergent species of bacteria. We propose to name these as Group III Cpns because of their distant relatedness to both group I and II Cpns. The Group III lineage may represent a progenitor of the group II CPNs. (Techtmann and Robb, 2011).
We have been studying the structural basis of intrinsic stability of Group II Cpn60 chaperonins. We discovered that a highly charged C terminal domain determines the stability and themoactivity of Cpn60 in thermophiles (Luo et al, 2011). The mechanism of thermostabilization and the action of chaperonins is considerably simpler in Archaea than in Eukarya, particularly in hyperthermophiles suggesting that the former could represent a model system for eukaryotic chaperonins.
Techtmann, S, F.T. Robb (2010) Archaeal-like chaperones in bacteria. Proc. Natl. Acad. Sci USA. 107 (47) 20269- 20274.
Luo, H, Robb FT. (2011) A modulator domain controlling thermal stability in the Group II chaperonins of Archaea. Arch Biochem Biophys. 512(1):111-8.