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Biochemistry Seminar: Michael Hampsey, Rutgers - UMDNJ

  • Date
    Wed, Apr 23

    Time
    12:00 PM — 1:00 PM

    Address
    Marshak
    City College of New York
    160 Convent Ave

    Location
    Marshak, MR 1027

    p: 212.650.8803

    Admission
    Free

  • Event Details

    Michael Hampsey, Prof., Rutgers,  will give a talk on "Gene Loops Facilitate mRNA Export"

    Michael Hampsey, Prof., Dept of Biochemistry & Mol Biology, Rutgers-UMDNJ, "Gene Loops Facilitate mRNA Export"


    ABSTRACT:

    Gene loops juxtapose the promoter and terminator regions of genes in yeast and mammalian cells.  Gene loops are formed on RNA Pol II-transcribed genes and are dependent upon the transcription initiation factor TFIIB and components of the pre-mRNA 3’-end processing machineries.  We are engaged in a systematic analysis of the genetic and physical requirements for looping.  Looping is RNA-dependent, requires specific components of the TREX-1 and TREX-2 complexes that couple transcription with mRNA export, and occurs prior to translocation of loops to the nuclear pore complex.  Earlier work from Hannah Klein’s laboratory identified defects in TFIIB (sua7/soh4) as suppressors of the growth defect associated with deletion of the Hpr1 component of the TREX-1 complex (Fan, HY, Cheng, KK and Klein, HL (1996) Genetics, 142:749-59).  We find that the hpr1Δ mutant is defective for both loop formation and mRNA accumulation in the cytoplasm.  Moreover, the sua7/soh4 suppressors restore looping as well as mRNA export.  We propose a model whereby gene loops interact with the nuclear pore complex to facilitate export of nascent mRNA to the cytoplasm.
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