Mirkó Palla, Postdoctoral Research Fellow, Department of Genetics, Harvard Medical School, Wyss Institute for Biologically Inspired Engineering, MA, will give a talk titled, "High-Throughput Nanopore-Based Methods to Read DNA"

ABSTRACT

Driven by the maturation of next-generation sequencing technologies, DNA sequencing has been dramatically expanding its scope in basic life science research and in clinical medicine. In this seminar, I will detail our recent study (Stranges* and Palla* et al., PNAS, 2016), in which we have provided proof-of-principle for a new integrated sequencing system that can lay the foundation for a highly scalable, accurate, single-molecule DNA sequencing platform. This has the potential to sequence entire genomes at much lower costs and to become instrumental for transforming many areas of precision medicine. I will then discuss an extension of this platform technology used as a multiplex screening tool for a library of DNA polymerases (in revision: Palla* and Punthambaker* et al., Nature Biotechnology, 2018) to identify variants with novel properties for applications of biotechnological interest.