Biochemistry Seminar: Joseph Marcotrigiano
Wed, Sep 03
12:00 PM — 1:00 PM
MarshakCity College of New York160 Convent Avenue
"Structural insight into hepatitis C virus infection and the innate immune response"Joseph Marcotrigiano, Associate Professor, Dept of Chemistry & Chemical Biology, Rutgers University, will give a talk on "Structural insight into hepatitis C virus infection & the innate immune response."
The Marcotrigiano laboratory, at the Center for Advanced Biotechnology and Medicine/ Rutgers University, is studying hepatitis C virus (HCV) entry, replication and host responses to infection. Worldwide, more than 160 million individuals are chronically infected with HCV. At present, there is no effective vaccine against HCV or entry inhibitor. Both vaccine development and entry inhibitors depend critically on successful structural and mechanistic studies of HCV surface glycoproteins and their interactions with obligate cellular receptors. Until recently such work has been hampered by the challenges inherent in preparing homogenous samples of disulfide rich proteins with complex, post-translational modifications (PTMs). To overcome this hurdle, we developed a cost-, time-, and labor-efficient mammalian cell expression system capable of producing milligram quantities of such proteins with a variety of PTMs. Our technology development efforts recently enabled successful crystallographic and mechanistic studies of HCV envelope glycoprotein E2 (Khan et al. Nature, 2014). In addition, we have determined the mechanism of self vs non-self differentiation of RNA molecules by a critical, cellular innate immune receptor (RIG-I, retinoic-acid-inducible gene-I) (Saito et al. Nature, 2008; Jiang et al. Nature, 2011) and have established a new mechanism of viral polyprotein processing and replication (Shin et al PNAS, 2012). Our long-term goal is to understand the interactions between HCV and host, paving the way towards developing novel antiviral drugs, vaccines, and immunomodulators.