Kevin Ryan

Professor (Biochemistry Division)

Program Director, Biochemistry Masters Degree Program (CCNY). Department Biochemistry Sub-division Chair. Campus-wide Radiation Safety Committee Chair.

Main Affiliation

Chemistry and Biochemistry

Additional Departments/Affiliated Programs

Graduate Center

Areas of Expertise/Research

  • RNA
  • Olfaction Flavors and Fragrances
  • Biochemistry

Building

Center for Discovery and Innovation

Office

4.304

Phone

212-650-8132

Fax

212-650-6107

Kevin Ryan, Professor

Kevin Ryan

Education

Ph.D. in Chemistry and Biochemistry, University of Rochester,1996 (Thesis advisor: Eric Kool).
Post-doc, Columbia University, Chemistry, 1996-1999 (with W. Clark Still).
Post-doc, Columbia University, Biology, 1999-2003 (with James L. Manley).

B.S. Chemistry, Providence College, Providence, RI.

Recent Teaching

Chem 32002 Biochemistry I (CCNY).
Chem 21000 Applied Chemistry for Biomedical Engineers (CCNY).
Chem A8208 RNA Biochemistry and Molecular Biology (CCNY).
BICM 75000 BioOrganic Chemistry (CUNY Graduate Center Biochemistry PhD Program).
CHEM U80541 Adv. Seminar in Molecular Biophysics (CUNY Graduate Center Chemistry PhD Program).

Chem B9800 Biochemistry Seminar (CCNY).

Current Research Interests

Olfactory molecular recognition and the mammalian odorant (GPCR) receptors.

Chemically synthesized small RNA cellular delivery vectors.

Biochemistry & Medicinal Chemistry of Myodesopsia (collagen-hyaluronan vitreous humor opacities)

Publications

Conformational sensing by a mammalian olfactory receptor, M.T. Liu, M. Na, Y. Li, M. Biscoe and K. Ryan*, (under peer review)

Small RNA-seq: The RNA 5’ end ligation problem and a method to circumvent it,” L. Lama, J. Cobo, D. Buenaventura and K. Ryan,* Journal of Biological Methods 2019, Vol. 6(1) e108,  DOI: 10.14440/jbm.2019.269

“Single-neuron comparison of the olfactory receptor response to deuterated and non-deuterated odorants,” Mihwa Na, Min Ting Liu, Minh Q. Nguyen and Kevin Ryan*, ACS Chemical Neuroscience. 2019, 10, 1, 552-562 DOI: 10.1021/acschemneuro.8b00416, Published Oct. 22, 2018.

“Carbon chain shape selectivity by the mouse olfactory receptor OR-I7,” Min Ting Liu, Jianghai Ho, Jason Liu, Radhanath Purakait, Uriel N. Morzan, Lucky Ahmed, Victor S. Batista, Hiroaki Matsunami,* and Kevin Ryan* Organic and Biomolecular Chemistry, 2018, 16, 2541-2548. PMCID: PMC6067823.

“Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract,” Na, M., Valente, S. and Ryan, K.* Methods in Molecular Biology (2017) Eukaryotic Transcription and Post-Transcription Gene Expression Regulation Vol. 1507, Chapter 14 (Springer Publishing ISBN 978-1-4939-6516-8) November 2016

“RNA polymerase III initiation on coligo DNA templates containing loops of variable sequence, size and nucleotide chemistry,” Joy Patel, Lodoe Lama, Niklas Hoffmann, Jose Cobo Christoph Müller and Kevin Ryan,* Gene (2016) 612:49-54 Epub September 2, 2016, http://dx.doi.org/10.1016/j.gene.2016.09.001

“An investigation into the role of ATP in the mammalian pre-mRNA 3’ cleavage reaction,” Khleborodova, A., Nagre, N., Pan, X., and Ryan, K.* Biochimie 2016 (April 6) 125: 213-222. PMID 27060432. DOI: 10.1016/j.biochi.2016.04.004

“Adenylylation of small RNA sequencing adapters using the TS2126 RNA ligase I,” Lodoe Lama and Kevin Ryan,* RNA 2016 Jan; 22(1):155-61. doi: 10.1261/rna.054999.115. Epub 2015 Nov 13. PMID: 26567315; PMCID: PMC4691829

“Aldehyde Recognition and Discrimination by Mammalian Odorant Receptors via Functional Group-Specific Hydration Chemistry,” Li Y, Peterlin Z, Ho J, Yarnitzky T, Liu MT, Fichman M, Niv MY, Matsunami H, Firestein S,* Ryan K.* ACS Chemical Biology. (2014) Vol. 9 (11): 2563-71; PMID: 25181321; PMCID: PMC4245160.

“New insights into the promoterless transcription of DNA coligo templates by RNA polymerase III,” Lodoe Lama, Christine I. Seidl and Kevin Ryan,* 2014 Transcription, Vol. 5, Issue 2. Published online 27 January 2014; http://dx.doi.org/10.4161/trns.27913; PMID: 24531370 

“Analysis of RNA processing reactions using cell free systems: 3' end cleavage of pre-mRNA substrates in vitro,” Joseph Jablonski, Mark Clementz, Kevin Ryan and Susana T. Valente,* J Vis Exp. (JoVE), 2014 May 3, (87). doi: 10.3791/51309.

“Structurally diverse low molecular weight activators of the mammalian pre-mRNA 3’ cleavage reaction,” Min Ting Liu, Nagaraja N. Nagre, and Kevin Ryan,* Bioorganic and Medicinal Chemistry 2013 Jan 15; 22(2):834-41. doi: 10.1016/j.bmc.2013.12.006. Published online 2013 Dec. 15. PMID: 24373842

“Circularized synthetic oligodeoxynucleotides serve as promoterless RNA polymerase III templates for small RNA generation in human cells,” C.I. Seidl, L. Lama and K. Ryan*, 2012 Nucleic Acids Research 2013 41 (4): 2552-2564. doi: 10.1093/nar/gks1334 First published online: December 28, 2012. PMCID: PMC3575851

“Circular Single-Stranded Synthetic DNA Delivery Vectors for microRNA,” C. Seidl and K. Ryan*, PLoS One, Feb. 2011 6(2): e16925. doi:10.1371/journal.pone.0016925. PMCID: PMC3040211

“Discrimination of saturated aldehydes by the rat I7 olfactory receptor,” M.D. Kurland, M.B. Newcomer, Z. Peterlin, K. Ryan, S. Firestein, and V.S. Batista*, Biochemistry, 2010, 3, 49(30):6302-4. PMCID: PMC2912408

“Molecular Shape and the Sense of Smell,” Kevin Ryan* and Xiaozhou P. Ryan, book chapter in The 2010 McGraw-Hill Yearbook of Science and Technology, 2010, 243-245.

“Small-molecule activators of pre-mRNA 3’ cleavage,” Kevin Ryan*, Asya Khleborodova, Jingyi Pan, and Xiaozhou P. Ryan RNA, 2009 15, 3, 483-492. PMCID: PMC2657006

“The Importance of Odorant Conformation to the Binding and Activation of a Representative Olfactory Receptor,” Zita Peterlin, Yadi Li, Grant Sun, Rohan Shah, Stuart Firestein, and Kevin Ryan* Cell Chemical Biology (formerly Chemistry & Biology), 2008 15, 12, 1317-1327.

“Finishing Touches: Post-Translational Modification of Protein Factors Involved in Mammalian pre-mRNA 3’ End Formation,” Kevin Ryan* and David L.V. Bauer, Int. J. Biochem. & Cell Bio. 2008 40, 11,2384-2396.

“Pre-mRNA 3’ Cleavage is Reversibly Inhibited in Vitro by Cleavage Factor Dephosphorylation,” Kevin Ryan*, RNA Biology, 2007, 4 (1) 26-33.

“Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation,” Daron Barnard, Kevin Ryan, James L. Manley and Joel Richter*, Cell, 2004, 119 (5), 641-51.

“Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease,” Kevin Ryan, Olga C. Calvo and James L. Manley*, RNA, 2004, 10 (4):565-73.

“Requirements of the RNA Polymerase II C-Terminal Domain for Reconstituting pre-mRNA Cleavage,” Kevin Ryan, Kanneganti G.K. Murthy, Syuzo Kaneko and James L. Manley*, Molecular and Cellular Biology, 2002, 22, 6, 1684-1692.

“Phosphorylation of CPEB by Eg2 Mediates the Recruitment of CPSF into an Active Cytoplasmic Polyadenylation Complex,” Raul Mendez, Kanneganti G.K. Murthy, Kevin Ryan, James L. Manley and Joel D. Richter,* Molecular Cell, 2000, Nov; 6(5):1253-9.

“A Synthetic Receptor Motif Designed for Extended Peptide Conformations,” Kevin Ryan, Leland Gershell, and W. Clark Still,* Tetrahedron, 2000, 21, 3309-3318.

“Inhibition of Gene Expression in Human Cells Through Small Molecule-RNA Interactions,” Seongwoo Hwang, Natarajan Tamilarasu, Kevin Ryan, Ikramul Huq, W. Clark Still, and Tariq M. Rana,* Proceedings of the National Academy of Sciences, USA, 1999, 96, 23, 12997-13002.

“Construction of Sequence-Selective Peptide Receptors from Conformationally Restricted Eta- and Theta- Amino Acids,” Kevin Ryan and W. Clark Still,* Bioorganic and Medicinal Chemistry Letters, 1999, 9, 2673-2678.

“Triplex-Directed Self-Assembly of an Artificial Sliding Clamp on Duplex DNA,” Kevin Ryan and Eric T. Kool,* Cell Chemical Biology (formerly Chemistry & Biology), 1998, 5:59-67.  Also featured on the cover of this issue.

“Synthesis and Reactivity of Pyrrolo[3,2,E]indole: Removal of an N-bom Group From an Unactivated Indole,” John E. Macor,* James T. Forman, Ronald J. Post and Kevin Ryan, Tetrahedron Letters, 1997, 38, 10, 1673-1676.

"A Direct Synthesis of 3-(Pyrrolidin-3-yl)indoles for Use As Conformationally Restricted Analogs of Tryptamines" John E. Macor, David H. Blank, Kevin Ryan, Ronald J. Post, Synthesis 1997, 443-449.

“Rolling Circle DNA Synthesis: Small Circular Oligonucleotides as Efficient Templates for DNA Polymerases,” Dongyu Liu, Sarah L. Daubendiek, Martin A. Zillman, Kevin Ryan, and Eric T. Kool,* Journal of the American Chemical Society, 1996, 118, 1587-94.

"Rolling-Circle RNA Synthesis: Circular Oligonucleotides as Efficient Substrates for T7 RNA Polymerase," Sarah L. Daubendiek, Kevin Ryan, and Eric T. Kool,* Journal of the American Chemical Society, 1995, 117, 7818-7819.

"5-(3-Nitropyrid-2-ylamino)indoles: Novel Serotonin Agonists with Selectivity for the 5-HT1D Receptor. Variation at C3 of the Indole Template Leads to Increased 5-HT1D Receptor Selectivity," John E. Macor,* David H. Blank, Carol B. Fox, Lorraine A. Lebel, Michael E. Newman, Kevin Ryan, Ronald J. Post, Anne W. Schmidt, David W. Shullz, and B. Kenneth Koe, Journal of Medicinal Chemistry, 1994, 37, 2509.

"The Synthesis of a Conformationally Restricted Analog of the Neurotransmitter             Serotonin with Reduced Basicity," John E. Macor and Kevin Ryan, Heterocycles, 1994, 37, 1447.

"A Simple Synthesis of 5-Amino-3-(2-dimethylaminoethyl) indole[5-amino-N,N-dimethyl-tryptamine]," John E.Macor,* Ronald J.Post, and Kevin Ryan, Synthetic Communications, 1993, 23, 65.

"Synthesis and Serotonergic Pharmacology of the Enantiomers of 3-[(N-Methylpyrrolidin-2-yl)methyl]-5-methoxy-1H-indole: Discovery of Stereogenic Differentiation in the Aminoethyl Side Chain of the Neurotransmitter Serotonin," John E. Macor ,* James Blake, Carol B. Fox, Celeste Johnson, B. Kenneth Koe, Lorraine A. Lebel, Jean M. Morrone, Kevin Ryan, Anne W. Schmidt, David W. Schulz, and Stevin H. Zorn, Journal of Medicinal Chemistry,1992, 35, 4503-4505.

"A Synthesis of 5-Carboxamidopyrrolo[3,2-b]pyridine," John E. Macor*, Ronald Post and Kevin Ryan, Journal of Heterocyclic Chemistry, 1992, 29, 1465-1467 .

"Synthesis of a Conformationally Restricted Analog of the Anti-Migraine Drug Sumatriptan," John E. Macor,* David H. Blank, Ronald J.Post and Kevin Ryan, Tetrahedron Letters, 1992, 33, 52, 8011-8014 .

"The Synthesis of Pyrano[3,2-e]indoles and Pyrano[2,3-f]indoles as Rotationally Restricted Phenolic Analogs of the Neurotransmitter Serotonin," John E. Macor*, Kevin Ryan, and Michael E. Newman, Tetrahedron, 1992, 48, 6, 1039-1052.

"Synthesis of Some Conformationally Restricted Serotonin Analogs," John E. Macor* and Kevin Ryan, Heterocycles, 1990, 31, 8, 1497-1504

"Synthesis of [3H]CP-93,129: A  Radioligand Selective for the 5-HT1B Receptor," John E. Macor,* Kevin Ryan, Phillip M. Chalabi, James C. Windels, and Robert W. Roth, Journal of Labelled Compounds and Radiopharmaceuticals, 1990 Vol.XXVIII, No.11, 1293.

"3-(1,2,5,6-Tetrahydropyrid-4-yl)pyrrolo[3,2-b]pyrid-5-one: A Potent and Selective Serotonin (5HT1B) Agonist and Rotationally Restricted Phenolic Analog of 5-Methoxy-3-(1,2,5,6-tetrahydropyrid-4-yl)indole," John E. Macor,* Carol A. Burkhart, James H. Heym, Jeffrey L. Ives, Lorraine A. Lebel, Michael E. Newman, Jann A. Nielsen, Kevin Ryan, David W. Schulz, Leslie K. Torgersen, and B. Kenneth Koe, Journal of Medicinal Chemistry, 1990, 33, 2087-2093.

"Synthesis of Pyrano[4,3-b]indoles as Conformationally Restricted Analogues of the Serotonin Antagonist ICS 205-930 and as Precursors to 2-Vinylindoles," John E. Macor,* Kevin Ryan, and Michael E. Newman, Journal of Organic Chemistry, 1989, 54, 20, 4785-4795.

"A Simple Synthesis of 2-Vinylindoles," John E. Macor,* Kevin Ryan and Michael E. Newman, Tetrahedron Letters, 1989, 30, 19, 2509-2512.