M. Lane Gilchrist
Faculty and Staff Profiles
M. Lane Gilchrist
Associate Professor
School/Division
Department
- Grove School of Engineering
- Chemical Engineering
Additional Departments/Affiliated Programs::
- Biomedical Engineering
Office
Steinman Hall, 306
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Group website
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Education
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Postdoctoral Fellow, Department of Chemistry, Columbia University. 1995-1999 (NIH NRSA Fellow 1997-1999)
Ph.D. in Biophysical Chemistry. University of California, Davis, Department of Chemistry, 1989-1995
B.S. in Chemical Engineering. Louisiana State University, 1981-1987
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Research Interests / Courses Taught
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Molecular Engineering of Proteins; Biomembranes and Membrane Proteins; Fluorescence Spectroscopy and Imaging; Magnetic Resonance Spectroscopy.
Supported Biomembrane Systems for Membrane Protein Studies, Biointerface Engineering of Phase Separated Lipid Microenvironments
Thermodynamics II; CHE 3300
Unit Operations I: Separations CHE 3450
Introduction to Bioprocess Engineering; CHE 5800
Advanced Chemical Engineering Thermodynamics CHE I2800
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Research Interests
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In Vitro Biomembrane Hybrid Systems
Biomembranes are amazing, multifunctional structures composed of a continuous bilayer of lipid molecules embedded with integral and peripheral membrane proteins. Membrane proteins make up ~30% of the genome of a typical eukaryotic cell, yet due largely to a strict requirement of a highly mobile, native-like biomembrane microenvironment, these molecules have not been widely built into functional materials.
Membrane protein structure is often highly complex, typified by large, multi-subunit complexes that not only span the lipid bilayer but also contain large (>2 nm) cytoplasmic and extracellular domains that protrude from the membrane. We have been researching the construction of stabilized biomembrane hybrid systems based on a biomimetic, surface-tethered artificial cytoskeleton where membrane-protein-polymer bioconjugates anchor the lipid bilayer and provide adequate biomembrane to substrate spacing. This application of this core technology is to enable in vitro supported biomembrane systems in which membrane proteins with large cytoplasmic domains can be stably interfaced with materials. Furthermore, the anchoring of biomembranes in this fashion could lead to assemblies that could hold up to shear, flow, and friction in challenging microenvironments such as found in microdevices, packed beds, and biomaterials.
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Selected Publications
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Google Scholar
https://scholar.google.com/citations?user=Jb4Ek7gAAAAJ&hl=en
Calhoun, D. H. and Gilchrist, M. L., Scavenger Receptor Uptake For Fabry Disease Enzyme Replacement Therapy. U.S. Patent Application US13970551, Patent awarded 11/28/18
Kyeyune-Nyombi, E., Morone, F., Liu, W., Li, S. Gilchrist, M. L.*, Makse, H. A.* (2017) High-resolution of particle contacts via fluorophore exclusion in deep-imaging of jammed colloidal packings. Physica A: Statistical Mechanics and its Applications (in press)
Fried, E. S., Li, Y. Gilchrist, M.L. (2017). Phase Composition Control in Microsphere-Supported Biomembrane Systems. Langmuir 33 (12), pp 3028–3039
Fried, E. S., Luchan, J. Gilchrist, M. L. (2016). Biodegradable, Tethered Lipid Bilayer-Microsphere Systems with Membrane-Integrated α-Helical Peptide Anchors. Langmuir, 32 (14), pp 3470–3475
Gilchrist, M. L., Ahn, K. and Li, Y.M. (2016). Imaging and Functional Analysis of γ-Secretase and Substrate in a Microsphere-Supported Biomembrane System. Analytical Chemistry 88(2), 1303-1311
Lin, H. A., Gupta, M. S., Varma, D. M. , Gilchrist, M. L., Nicoll, S. B., (2015) Lower crosslinking density enhances functional nucleus pulposus-like matrix elaboration by human mesenchymal stem cells in carboxymethylcellulose hydrogels. J Biomed Mater Res A. 104 (1), 165–177
Hume, J. , Sun, J, Jacquet, R., Renfrew, P. D., Martin, J. A., Bonneau, R., Gilchrist, M. L. and Montclare, J . K.. (2014) Engineered Coiled-Coil Protein Microfibers Biomacromolecules 15 (10), 3503–3510
Zhong, L., Tu, R. S. and Gilchrist, M. L. (2013) Tether-Supported Biomembranes with α‐Helical Peptide-Based Anchoring Constructs Langmuir 28, 299-307
Chen, X., Shojaei-Zadeh, S., Gilchrist, M. L., Maldarelli, C, (2013) A lipobead microarray assembled by particle entrapment in a microfluidic obstacle course and used for the display of cell membrane receptors Lab on a Chip 13, 3041-3060
Chau, D.M., Shum, D., Radu, C., Bhinder, B., Gin, D., Gilchrist, M. L., Djaballah, H., Li, Y.M. A Novel High-Throughput 1536-well Notch1 γ-Secretase AlphaLISA Assay.Comb Chem High Throughput Screen. 16 (6), 415
Ahn, K., Shelton, C. C., Tian, Y. M., Zhan, X, Gilchrist, M. L., Sisodia, S. S. and Li, Y-M, Intrinsic g-Secretase and Presenilinase Activity of Presenilin 1(2010) Proc Natl Acad Sci U S A 107 (50) 21435-21440;
Vaidya, S., Gilchrist, M. L., Maldarelli, C. M., and Couzis, A, (2007) Spectral Barcoding of Polystyrene Microbeads Using Multicolored Quantum Dots, Analytical Chemistry
Dudu, V., Ramcharan, M., Gilchrist, M. L., Holland, E.. C., and Vazquez, M, (2007) Liposome Delivery of Quantum Dots to the Cytosol of Live Cells Journal of Nanoscience and Nanotechnology
Sharma, M.K. and Gilchrist, M. L., (2007) Templated Assembly of Biomembranes on Silica Micropheres using Bacteriorhodopsin Conjugates as Structural Anchors, Langmuir 23, 7101-7112
Kalyankar, N. D., Sharma, M.K., Vaidya, S., Calhoun, D.H., Maldarelli, C. M., Couzis, A. and Gilchrist, M. L., (2006) Arraying of Intact Liposomes Into Chemically Functionalized Microwell Surfaces, Langmuir 22(12):5403-11
Vengrenyuk Y, Carlier S, Xanthos S, Cardoso L, Ganatos P, Virmani R, Einav S, Gilchrist L, Weinbaum S. A hypothesis for vulnerable plaque rupture due to stress-induced debonding around cellular microcalcifications in thin fibrous caps. (2006) Proc Natl Acad Sci U S A. Oct 3;103(40):14678-83
Sharma, M. K., Jattani, H., and Gilchrist, M. L., (2004) Bacteriorhodopsin Conjugates as Anchors for Supported Lipid Bilayers, Bioconjugate Chemistry 15(4); 942-947
Sampathkumar, P. and Gilchrist, M. L., (2004) Synthesis and Characterization of Bioconjugates of S-Layer Proteins, Bioconjugate Chemistry 15(4) 685-693
Britt, R. D., Campbell, K., Peloquin, J., Gilchrist, AM. L., Ansar, C., Dicus, M., Robbele, M., Messinger, J., (2004) Recent Pulsed EPR Studies of the Photosystem II Oxygen Evolving Complex: Implications as to Water Oxidation Mechanisms, Biochemica Et Biophysica Acta (BBA)-Bioenergetics 1655(1-3):158-71
M. Lane Gilchrist Jr., Monde, K. Tomita, Y.; Iwashita, T.; Nakanishi, K. and McDermott, A. E. (2001), Measurement of Interfluorine Distances in Solids. Journal of Magnetic Resonance, 152, 1-6
Gilchrist, M. L. Jr.; Randall, D. R.; Ball, J. A.; Britt, R. D. (1995), Proximity of the Redox Active Tyrosine Yz. to the Manganese Cluster of Photosystem II. Proceedings of the National Academy of Sciences, 92, 9545-9549
Tang, X.-S.; Diner, B. A.; Larsen, B. S.; Gilchrist, M. L. Jr.; Lorigan, G. A.; Britt, R. D. (1994), Identification of Histidine at the Catalytic Site of the Photosynthetic Oxygen Evolving Complex. Proceedings of the National Academy of Sciences , 91, 704-708