Biochemistry Seminar: Matthias Buck, "Dynamic protein-protein and protein-membrane complexes: A perspective from molecular dynamics simulations and solution NMR spectroscopy"

Dates
Tue, Sep 03, 2019 - 12:00 PM — Tue, Sep 03, 2019 - 01:00 PM
Event Address
ASRC
City College of New York
85 Saint Nicholas Terrace
Phone Number
212-650-8803
Event Location
ASRC Main Auditorium
Event Details

Matthias Buck, Professor, Department of Physiology and Biophysics, Case Western Reserve School of Medicine, OH, will give a talk titled, "Dynamic protein-protein and protein-membrane complexes: A perspective from Molecular Dynamics Simulations and solution NMR spectroscopy"


ABSTRACT


It is now recognized that protein-protein interactions in solution are often dynamic, especially if the binding affinities are only moderately strong. Dynamics originate in part from the interconversion between structures of the protein complex, e.g. one bound state that is in equilibrium with one or several alternate configurations. We determined the structure of such a complex using NMR restraints and saw the transitions between different configurations in microsecond length all-atom molecular dynamics simulations. Recently, we also studied the dissociation process of mutant complexes which had a weakened primary interaction interface. Those simulations suggested that there is no single dissociation pathway, but that the separation first involves transitions to binding interfaces with fewer/weaker contacts. Comparison is made between experimental NMR relaxation measurements on the ps-ns as well as µs-ms timescale with the microsecond all atom simulations, also in the context of new simulations of the protein association process. The functional significance of the protein complex alternate states and their dynamics are discussed.


In a second part of the presentation, we consider a second system involving transient interactions; this time between K-Ras and the lipid bilayer of the plasma membrane. Our recent simulations the full length GTPase at different membranes reveal the underlying rules of the interactions, emphasizing electrostatic contacts but also protein topology [5]. There is also extensive dynamics at the membrane of the K-Ras : cRaf complex and a novel strategy for inhibiting the Ras protein is suggested.


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