Pilar Cossio, Research Scientist and Project Leader for Structural and Molecular Biophysics at the Flatiron Institute Center for Computational Mathematics, will give a talk on "Free energies from cryoEM particles."

ABSTRACT

Cryo-electron microscopy (cryo-EM) is an experimental technique that measures single-particle projections of biomolecules. Although single-particle cryo-EM is widely used for 3D reconstruction, it has the potential to provide information about a biomolecule’s conformational variability and provide quantitative measurements of probability distributions. However, cryo-EM images are challenging to analyze due to their low signal-to-noise ratio. To address these issues, we developed mathematical methods to infer free-energy profiles and their uncertainties from cryo-EM raw images.