Biochemistry Seminar: Samuel Sternberg, "Evolutionary and mechanistic diversity of CRISPR RNA-guided transposases"

Wed, Dec 08, 2021 - 12:00 PM — Wed, Dec 08, 2021 - 01:00 PM
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85 Saint Nicholas Terrace
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via Zoom
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ASRC Main Auditorium:
* Current Cleared4 Pass or CCNY ID with gold V22 sticker required for entrance
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via Zoom
Event Details

Meeting ID: 495 404 8198


Samuel Sternberg, Assistant Profssor, Dept. of Biochemistry & Molecular Biophysics, Columbia University Medical Center, will give a talk on "Evolutionary and mechanistic diversity of CRISPR RNA-guided transposases."


Conventional CRISPR–Cas systems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mobile genetic elements, including plasmids and viruses. In a remarkable inversion of this paradigm, bacterial transposons have coopted nuclease-deficient CRISPR–Cas systems to catalyze RNA-guided integration of mobile genetic elements into the genome. Here we show that programmable transposition occurs at a fixed distance downstream of target DNA sequences, accommodates variable length genetic payloads, and functions robustly in diverse bacterial species. Deep sequencing experiments reveal highly specific, genome-wide DNA integration, which is enabled by the coordinated and sequential recruitment of transposase factors to target sites specified by Cascade. By exploring a large set of evolutionarily diverse CRISPR-transposon systems, we further define key sequence motifs that establish transposase-transposon specificity during DNA excision and integration. The discovery of a fully programmable, RNA-guided transposase lays the foundation for kilobase-scale genome engineering that obviates the requirements for DNA double-strand breaks and homologous recombination.


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